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KMID : 0378019850280060040
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New Medical Journal
1985 Volume.28 No. 6 p.40 ~ p.46
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Interactions of Aspirin, Indomethacin and Furosemide on the Renal Tissue Levels of Cyclic Nucleotide in Rats
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이석용/Lee, S.Y.
이권행/김옥녀/이상복/조규철/--/Kim, O.N./Lee, S.B./Cho, K.C.
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Abstract
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Recent studies, which were designed to define the role of renal prostaglandins as a possible mediator of the effects of diuretics, show that aspirin or indomethacin significantly decreases the diuretic and natriuretic response of furosemide (Berg & Law, 1977; Chennavasin et al., 1930). However, it is not clear whether the mechanism of the inhibitory effect of aspirin or indomethacin on the diuretic action of furosemide is solely due to the interference of prostaglandin synthesis.
Since prostaglandins are known as potent stimulator of the synthesis of cAMP in various tissues (Collier & Roy, 1974; Sharma et al., 1975), it is rational to assume that aspirin or indomethacin may influence on the renal tissue level of cyclic nucleotide in the furosemide treated animal.
The renal tissue levels of cAMP and cGMP were assayed by binding test with cAMP or cGMP radioimmunoassay kits (Amersham, England) in control, 10, 30 and 60 min after administration of aspirin (10 mg/kg), indomethacin (2.5 mg/kg), furosemide (1 mg/kg) and after combined administrations of furosemide with either aspirin or indomethacin in the Sprague-Dawley rats.
The results were as follows;
1. The renal-tissue levels of cAMP were significantly increased 10 min after administration of aspirin and returned to control level after 30 min; however, cGMP level was significantly decreased after drug administration and returned to control level at 60 min.
2. The renal tissue levels of cAMP were not changed significantly after administration of indomethacin. However, the renal tissue levels of cGMP were significantly decreased following indomethacin injection and returned to control level after 60 min.
3. The renal tissue levels of cAMP were not changed significantly following administration of furosemide, and the renal cGMP levels were significantly increased at 10 and 30 min after furosemide injection returned to control level at 60 min.
4. The renal tissue levels of cAMP were not changed significantly following administr ations of furosemide with either aspirin or indomethacin. The renal tissue levels of cGMP was significantly ´ increased 10 min after administrations of furosemide with aspirin. Combined administrations of furosemide. and indomethacin did not altered renal cGMP level.
The results of this experiment indicate that the interference of aspirin or indomethacinn on. the furosemide action may be related to the renal tissue level of cGMP rather than CAMP level.
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